Home > Services > 6. Analytical services > 6.2 Peptide Mapping by LC-MS/MS
6.2 Peptide Mapping by LC-MS/MS
Peptide mapping by liquid chromatography-mass spectrometry
Note: Price/availability/specifications subject to change without notice. All products and services are for research use only and not intended for use in humans.
Order offline: Fax PO to 650-654-3320 or call 650-288-4891 or email info@lakepharma.com
Features:
Peptide mapping by LC-MS/MS can be used to determine detailed information about the peptide's sequence and post-translational modifications.
Description:
• Deliverable information:
- Confirmation of primary sequences (sequence coverage: 60-90% using one enzyme; > 98% using two or more enzymes)
- Glycoprofiling of N-linked oligosaccharides (heterogeneity of glycoforms & approximation of site occupancy)
- Identification & approx quantification of PTMs such as deamidation, oxidation, N-term pyroglutamate formation and C-term Lys processing (modified amino acid position and % modification can be detected)
- Similarity assessment between different lots
- Comparability assessment for biosimilars
• Sample treatment: Denaturation, reduction/alkylation and enzymatic digestion followed by LC-MS or LC-MS/MS
• Sample requirement: 100 – 200 μg
• Turnaround time: 2 - 3 weeks
Related Links:
