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scFv engineering
Updated: February 01, 2011
Single chain variable fragment (scFv) is a fusion of the variable regions of the heavy (VH) and light chains (VL) of immunoglobulins, connected with a short linker peptide of 10 to 25 amino acids. Made as a single protein, scFv-Fc retains the specificity of the original immunoglobulin, and in many applications outperforms whole IgG in construction speed, production yield, and engineering flexibility.
LakePharma utilizes scFv in its antibody cloning and antibody optimization platforms. The company has developed a highly efficient process to engineer scFv-Fc fusions and produce the proteins in CHO cells. The scFv-Fcs generally retains the binding affinity and specificity as whole IgGs, and can be used to perform antigen binding confirmation during antibody cloning. scFv format is also used in molecular evolution for antibody affinity maturation. With its optimized scFv expression vectors and its proprietary scFv construction technologies, LakePharma leads the industry in constructing and producing scFvs.
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