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Protein engineering
Updated: March 17, 2010
LakePharma has extensive technical expertise in engineering native proteins and producing new versions of native proteins to obtain desirable properties. Below is our list of favorite engineering activities:
1. Cell secretion signal sequence
Secretion signal sequence or signal peptide is a short peptide sequence that directs the transport and cellular secretion of a protein in the classical pathway. Adding a signal sequence to some intracellular proteins can result in the secretion of the proteins. Replacing existing signal sequence with an optimal sequence often results in enhanced protein production and secretion.
2. Cell surface display
Just like we can convert intracellular proteins into secreted molecules, LakePharma can convert secreted proteins or intracellular proteins into cell surface proteins using its cell surface display platform.
3. Cell entry signal
Adding a cell entry signal to an excellular protein can effectively enable cell entry or uptake of that protein.
4. Chimeric molecule
Fusing two different molecules together is a classic protein engineering art. LakePharma now does it with aid of computer-based structural information.
5. Dominant negative
Dominant negative was a term originated from simple organism genetics, and was a great way of dissecting biological pathways. Dominant negatives are now used in protein engineering, by parsing a protein in a correct way, dominant negatives can be generated efficiently and reliably. They can be used as research reagents or even as future therapeutics.
6. Fc fusion
Attachment of Fc to peptides and proteins is a fantastic way to not only increase the half life of proteins and peptides in vivo, but also simplify purification process and molecular detection.
7. Molecular evolution
We apply molecular evolution to increase or decrease biological activities (i.e. binding affinity, catalytic activity). It is done with amino acid substitutions, either with computer algorithm-based functional prediction or random mutagenesis followed by functional screening.
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