Catalog #61200 - Sequence Confirmation by Trypsin Digestion (1 digestion)

Sequence Confirmation by Trypsin Digestion (1 digestion)


Sequence Confirmation by Trypsin Digestion (1 digestion)


1. The antibody/protein of interest is denatured, reduced and digested with one or more proteolytic enzymes, such as trypsin.

2. The resulting peptide mixtures are separated on a reverse-phase HPLC coupled to a mass spectrometer

3. The mass spectra is compared against the target protein sequence and the percent coverage is calculated.

Note: the above quoted prices are based on analysis using LakePharma’s standardized methods, proven to work for a majority of antibodies and proteins. Method development may be necessary for atypical proteins with unique properties for an additional fee.


Study report.

Material Required:
  • ≥ 100µg of purified protein at  1mg/mL
  • Target protein sequence
  • (Optional) 10mL blank buffer formulation
  • Please complete and include a Bioanalytical Sample Submission Form with any proteins or antibodies you send to LakePharma for analysis

1-2 weeks