Antibody Affinity Maturation

At LakePharma, different antibody affinity maturation methodologies using phage and yeast surface display platforms followed by high-throughput (HT) screening are employed to increase target binding affinity.

        1. CDR randomization of different Ab fragment scaffolds (scFv, Fab, VHH)

        2. Large single or large combination variable heavy/light chain yeast or phage display CDR libraries can be generated

        3. Multiple rounds of panning selections with increasing stringency can be performed in order to achieve demanding affinity improvements

LakePharma can generate light chain shuffling antibody libraries including yeast or phage display libraries via amplification of VL pools from B cells derived from human donors.

We offer antibody kinetic characterization using state-of-the-art instrumentation:

• Carterra® LSA array-based SPR for HT kinetic characterization of unpurified bacterial extracts enables early lead prioritization
• Carterra® LSA or BLI-based Octet used for IgG candidate kinetic characterizations

LakePharma's Affinity Maturation Services:

Client Testimonial: Tri-Institutional Therapeutics Discovery Institute and LakePharma: A Partnership in Antibody Discovery to Enable Innovative TherapeuticsClick here to download the free eBook: Introduction to Antibody Display-based Libraries

Download our Antibody Discovery and Engineering Services Brochure by clicking here. 

Contact Customer Support