- Molecular Biology
- Viral Vectors & Cell Engineering
- Protein Engineering & Production
- Antibody Discovery & Engineering
- Antibody Production
-
Analytics & Formulation
- Overview
- Purity Analysis by CE-SDS
- Aggregation Analysis
- Charge Variant Analysis
- De Novo Antibody Sequencing
- Intact Mass Analysis
- Sequence Confirmation by Mass Spectrometry
- N-terminal Sequencing
- Post-Translational Modification
- N-glycan Analysis
- Thermostability Analysis
- Stability Analysis
- Formulation Development
- Developability Analysis
- FAQs
- R&D and cGxP Bioassay Development
- Protein Chemistry
- Development & Manufacturing in LakePharma Hayward, CA
- Development & Manufacturing in LakePharma Hopkinton, MA
- Consulting Services
Antibody Affinity Maturation
At LakePharma, different antibody affinity maturation methodologies using phage and yeast surface display platforms followed by high-throughput (HT) screening are employed to increase target binding affinity.
1. CDR randomization of different Ab fragment scaffolds (scFv, Fab, VHH)
2. Large single or large combination variable heavy/light chain yeast or phage display CDR libraries can be generated
3. Multiple rounds of panning selections with increasing stringency can be performed in order to achieve demanding affinity improvements
LakePharma can generate light chain shuffling antibody libraries including yeast or phage display libraries via amplification of VL pools from B cells derived from human donors.
We offer antibody kinetic characterization using state-of-the-art instrumentation:
- Carterra® LSA array-based SPR for HT kinetic characterization of unpurified bacterial extracts enables early lead prioritization
- Carterra® LSA or BLI-based Octet used for IgG candidate kinetic characterizations
LakePharma's Affinity Maturation Services:
Client Testimonial: Tri-Institutional Therapeutics Discovery Institute and LakePharma: A Partnership in Antibody Discovery to Enable Innovative TherapeuticsClick here to download the free eBook: Introduction to Antibody Display-based Libraries
Download our Antibody Discovery and Engineering Services Brochure by clicking here.
Catalog # | Name | Timeline | Price |
---|---|---|---|
24110 | Affinity Maturation | 4 to 6 months | Request |