CHO Transient Production (TunaCHO™ Process)
CHO cells have been the workhorse for protein and antibody manufacturing. The major advantages of CHO include robust growth, hardiness in suspension culture, stellar protein production and secretion, and a long track record. The biggest drawback of CHO is that the cells are hard to transfect. As such, CHO cells have been primarily used for CHO stable production, not transient.
TunaCHO™ technology platform solves the technical problem of low transient transfection efficiency. We are able to achieve a production yield greater than 1 gram per liter in shake flasks. We further have shown consistent PTM profiles between TunaCHO™ and CHO-GSN™. Based on yields, Curia favors CHO over HEK293 as a transient protein production platform.
Below is a graph of typical yields between our Tuna293 transient production platform, our TunaCHO expression system, and other CHO transient expression system to show the difference in yields.
Recombinant antibody expression with TunaCHO™ process is significantly higher than HEK293 cells.
A variety of purification methods and production scales are available. Sample pricing is shown below.
| Catalog # | Name | Timeline | Price |
|---|---|---|---|
| 1335 | 0.1 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification | 3 weeks | $2,730 |
| 1336 | 0.25 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification | 3 weeks | $4,450 |
| 1338 | 1.0 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification | 3 weeks | $9,480 |
| 1738 | 0.03 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification | 2 weeks | $1,200 |
| 1739 | 0.1 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification | 2 weeks | $1,880 |
| 1740 | 0.25 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification | 2 weeks | $3,240 |
| 1742 | 1.0 L transient production in CHO cells (TunaCHO™ standard 7-day process) and Protein A purification | 2 weeks | $7,400 |
| 1788 | 0.03 L transient production in CHO cells (TunaCHO™ extended 14-day process) and Protein A purification | 3 weeks | $1,820 |
To learn more about these services, contact us to discuss your needs with an expert or fill out one of our Sample Submission forms.
Large Scale Antibody Production: Hybridoma or recombinant?
After successful identification of a unique hybridoma for your therapeutic or diagnostic program, you need toquickly generate gram quantities for further evaluation.
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