Catalog #1528 - High-throughput production service 96-well (TunaCHO™ 14-day process)
Title:
High-throughput production service 96-well (TunaCHO™ 14-day process)
Description:
High-throughput molecular construction (synthesize variable regions), DNA scale-up, 0.01 L transient production in CHO cells (TunaCHO™ 14-day process), and Protein A purification
DNA sequence confirmation is included
Intact mass QC by mass spectrometry is included for standard human and mouse IgGs only
Scope:
Molecular construction of IgG expression constructs in Curia’s proprietary vector (synthesize variable regions)
DNA scale-ups (midiprep scale), including sequence confirmation
0.01 liter transient productions in CHO cells (HTP TunaCHO™ process)
HTP Protein A purification
Intact mass spec QC (reduced and deglycosylated conditions), complimentary for standard human and mouse IgGs only
Deliverable:
All purified protein in standard buffer and unadjusted concentration obtained from 0.01 liter productions, frozen in 96-well deep well plate
Study Report
Summary of yields and aliquots
Mass Spec report
Additional service options:
Individual aliquots: $1200/plate
Material Required:
1. VH and VL sequences for 96 antibodies.
2. DNA template (if DNA synthesis is not desired).
3. Specified constant region for all 96 antibodies.
Note: One constant region for heavy chain and light chain must be specified for all 96 antibodies.
Timeline:
6-7 Weeks
Price:
Request