Antibody Production

LakePharma has extensive experience with constructing and producing antibodies in our mammalian platforms. Antibody variable region genes (VH and VL) from a given antibody can be reformatted to encode a different constant region and other formats including Fab, scFv, IgM, nanobody, and bispecific molecules. Multiple species and isotypes are available, with the option of synthesizing the constant region of choice.

These expression constructs can be expressed in HEK293 and CHO cells followed by enrichment and purification.  The molecule of choice can be rapidly produced at scales ranging from milligram to gram quantities. We also offer high throughput 96-block antibody productions to fit your antibody screening needs.

Download flyer: Large-scale Ab Production: Hybridoma or Recombinant?

Click here to learn more about TunaCHO for high yield antibody production.

Click here for an overview of gene-to-antibody and high-throughput antibody services.

New - Intact Mass Spec Analysis for All Recombinant Antibody Productions

High resolution mass spectrometers have achieved significant resolving power for large molecules. At LakePharma, we apply this unparalleled analytical capability to all recombinant antibodies we produce for our clients to enhance the quality standard, for peace of mind.

Additional services, such as peptide mapping and PTM analysis, are also available. Click here for a full list of analytical services available at LakePharma. 

New - in vitro Glyco Engineering (IVGE)

Glycosylation is a common type of post-translational modification that can significantly influence protein stability, biological activity, and pharmacokinetics of the final product. One way to manipulate glycosylation is through IVGE. 

LakePharma is teaming up with Roche Custom Biotech to offer custom IVGE service designed to improve therapeutic protein productions. With a two-step reaction of galactosylation followed by sialyation, glycoforms can be enriched with known kinetics and predictable outcomes. Click here to learn more details. 

"LakePharma was a great partner; very helpful, focused, and committed. Thank you for your antibody engineering and production services which made this project a success."

- Maria Day, ImmuNext

LakePharma is proud to contribute to ImmuNext's success.
View their press release here