- Molecular Biology
- Viral Vectors & Cell Engineering
- Protein Engineering & Production
- Antibody Discovery & Engineering
- Antibody Production
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Analytics & Formulation
- Overview
- Purity Analysis by CE-SDS
- Aggregation Analysis
- Charge Variant Analysis
- De Novo Antibody Sequencing
- Intact Mass Analysis
- Sequence Confirmation by Mass Spectrometry
- N-terminal Sequencing
- Post-Translational Modification
- N-glycan Analysis
- Thermostability Analysis
- Stability Analysis
- Formulation Development
- Developability Analysis
- R&D and cGxP Bioassay Development
- Protein Chemistry
- Development & Manufacturing in LakePharma Hayward, CA
- Development & Manufacturing in LakePharma Hopkinton, MA
- Consulting Services
Tissue Processing, Sample Prep and QC
A majority of experimental endpoints require the efficient manipulation and processing of Tissues, Cells, Biofluids and other experimental materials for total Protein, DNA/RNA, or Membrane Preparations. LakePharma provides standard and fully customized sample processing to meet client-specific needs and produce exceptional quality materials for downstream assay processes or delivery to client.
Highlights:
- Multiple Dissociation Modalities based on sample type and downstream application
- Qiagen Tissuelyser II and Roche MagNA Lyser Bead Mills, Stainless/Ceramic sterile beads
- Diagenode Bioruptor 300
- Multiple Dounce homogenization volumes
- Traditional probe sonnicator workflows
- Iodixanol and Glucose Gradient Ultracentrifuge Methods for Membrane Preparation and subcellular fractionation at a variety of volumes
- Agilent TapeStation 4200 instrument for RIN and DV200 analysis of samples
- NGS/RNAseq Sample preparation workflows and Quality Control on NGS input materials. Multiple available NGS Sequencing Partners for Data Delivery to Client Informatics Teams
- Broad team experience with dissection and parallel processing of large numbers of clinical study samples.
- Expertise across all animal tissue types
- Significant team experience in protein preparation methods for downstream LC/MS analysis
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