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Catalog #25120 - Cloning into expression plasmid with 1 liter expression (18 or 37 degrees C) and tandem affinity purification using Ni and streptactin resins
Title:
Cloning into expression plasmid with 1 liter expression (18 or 37 degrees C) and tandem affinity purification using Ni and streptactin resins
Description:
Cloning into expression plasmid with 1 liter expression (18 or 37 degrees C) and tandem affinity purification using Ni and streptactin resins
Scope:
1. Target gene(s) are cloned into LakePharma's proprietary bacterial expression plasmids with N or C-terminal His and/or Strep tags.
2. 1 liter expression in E. coli is performed at 18 oC or 37 oC with IPTG induction and the protein is purified by affinity chromatography using nickel resin or tandem affinity chromatography using nickel and streptactin resins. Elution fractions are analyzed by PAGE & Western blot and pooled based on purity metrics established with the client.
Please note: expression may be subsequently scaled up to 10 liters or more to produce protein quantities needed by clients.
Options available: endotoxin removal, affinity tag removal by protease cleavage (inquire), protein titer quantification (HPLC), analytical protein characterization including aSEC (HPLC), C-IEF, thermal stability analysis, mass-spec, and protein formulation optimization.
