Catalog #25610 - Cloning into expression plasmid, and P1 virus generation

Title:
Cloning into expression plasmid, and P1 virus generation

Description:
Cloning into expression plasmid, and P1 virus generation

Scope:

1. Target gene(s) are cloned into pFastBac expression plasmids (ThermoFisher) with N or C-terminal His and/or Strep tags. 

2. P1 virus (~30 ml at ~1.0x108 pfu/ml) is generated from plasmid by transfection of bacmid DNA into Sf9 cells.

3. If long term storage of the virus is required, a BIIC (Baculovirus Infected Insect cell) stock can be prepared.  

 Inquire for more information.


Deliverable:
1. P1 Virus 2. Purified protein 3. Study Report 4. Certificate of Analysis

Material Required:
Plasmid harboring ORF for target protein

Timeline:
4-5 Weeks

Price:
Request