Catalog #25620 - 30 ml small-scale expression analysis with affinity pulldown

Title:
30 ml small-scale expression analysis with affinity pulldown

Description:
30 ml small-scale expression analysis with affinity pulldown

Scope:

  

1. Small-scale expression analysis of the target protein is perfromed by infecting two cell lines with two dilutions of virus and harvesting the cultures at two viabilities (8 data points).  Affinity pulldowns are conducted from cell lysates or conditioned media and analyzed by PAGE and Western blot.  The condition with the highest level of recombinant protein is identified.  



Please note: production may subsequently be scaled up to 10 liters or higher to produce protein quantities needed by clients.

Options available: low endotoxin processing, affinity tag removal by protease cleavage, protein titer quantification (HPLC), analytical protein characterization including a SEC-HPLC, C-IEF, thermal stability analysis, mass-spec, and protein formulation optimization.

As an alternate to the Bac to Bac system, Lake Pharma is an authorized contract research manufacturer of pOET expression plasmids (Oxford Expression Technologies).  Inquire for more information.


Deliverable:
1. P1 Virus 2. Purified protein 3. Study Report 4. Certificate of Analysis

Material Required:
Plasmid harboring ORF for target protein

Timeline:
5-7 Days

Price:
Request