Catalog #25640 - 1 liter expression, and tandem affinity purification

Title:
1 liter expression, and tandem affinity purification

Description:
1 liter expression, and tandem affinity purification

Scope:

 

1. One liter expression in insect cells is performed and harvested at the expression conditions identified in the small scale expression analysis. The protein is purified by affinity chromatography using  tandem affinity chromatography using nickel and streptactin resins.  Elution fractions are analyzed by PAGE & Western blot and pooled based on purity metrics established with the client. 


Please note: production may subsequently be scaled up to 10 liters or higher to produce protein quantities needed by clients.

Options available: low endotoxin processing, affinity tag removal by protease cleavage, protein titer quantification (HPLC), analytical protein characterization including a SEC-HPLC, C-IEF, thermal stability analysis, mass-spec, and protein formulation optimization.

As an alternate to the Bac to Bac system, Lake Pharma is an authorized contract research manufacturer of pOET expression plasmids (Oxford Expression Technologies).  Inquire for more information.


Deliverable:
1. P1 Virus 2. Purified protein 3. Study Report 4. Certificate of Analysis

Material Required:
Plasmid harboring ORF for target protein

Timeline:
6-8 Days

Price:
Request